- \n
- Quick Facts<\/dt>\n
- NGS funded by the NSF<\/dd>\n
- The cutting edge next level of genetic sequencing to generate meta-data<\/dd>\n
- For sequencing of prepared NGS libraries<\/dd>\n
- Metagenomic reports generated with 16S analysis<\/dd>\n<\/dl>\n
The Miseq from illumina is used in cutting edge research and increasingly in healthcare for applications such as personalized medicine, IVF, cancer research. We can provide specialist advice if you have a project in mind and are not sure how to generate the data. In order to provide an estimate for cost per sample or per project please contact biology+tech@montclair.edu<\/a>. For 16S metagenomic analysis we will provide a pdf doc per sample as generated by the onboard program that compares the sequencing to the greengenes database<\/a>.<\/p>\n
View a sample report<\/a><\/span><\/p>\n
<\/div>\n![\"National](\"\/responsive-media\/cache\/biology\/wp-content\/uploads\/sites\/43\/2019\/06\/nsf.jpg.0.1x.generic.jpg\")
<\/figure>\nNGS research is funded by the National Science Foundation<\/p><\/div>
<\/div><\/p><\/div>\n<\/p><\/div><\/div>Capillary electrophoresis (Sanger) Sequencing with the Applied Biosystems 3130 Genetic Analyzer<\/div>\n- \n
- Quick Facts<\/dt>\n
- The gold standard for Genetic Sequencing<\/dd>\n
- Keywords: PCR product, plasmid<\/dd>\n
- Max length generated 750-850bp<\/dd>\n<\/dl>\n
Capillary electrophoresis (Sanger) Sequencing is still widely used and accepted as the gold standard of genetic sequencing due to its very high accuracy and has been used in research for the past 20 years. The process involves three stages once the sample is ready to be sequenced:<\/p>\n
- \n
- A cycle sequencing reaction where the fluorescently tagged nucleotides are incorporated into any DNA present by using a specific primer<\/li>\n
- The whole reaction must be cleaned up to remove all impurities<\/li>\n
- The sample is loaded onto the 3130 genetic analyzer where capillary electrophoresis produces an electropherogram which contains your genetic sequence.<\/li>\n<\/ol>\n
Read through the Applied Biosystems Sequencing Handbook<\/a> for information on our sequencer.<\/p>\n
Sequencing Samples Submission<\/h2>\n
General Requirements<\/h3>\n
Submit sequencing samples according to the instructions below:<\/p>\n
- \n
- Email biology+tech@montclair.edu<\/a>\u00a0a gel pic with the indicated bands and a positive and negative control if possible<\/li>\n<\/ul>\n
- \n
- Provide a labeled tube for the PCR product or plasmid (label plasmid tube with conc. in ng\/\u00b5L<\/em>) and a labeled tube for the forward and\/or reverse primer (do not combine primers<\/em>). Primer concentration should be 10\u00b5M<\/li>\n<\/ul>\n
- \n
- If you are setting up your own sequencing (ONLY for PCR) pre-mix. Use 1.5\u00b5L PCR, 1.5\u00b5L single Primer (At concentration of 10\u00b5M<\/em>) and 7\u00b5L water for a total of 10\u00b5L<\/li>\n<\/ul>\n
- \n
- Clearly indicate a unique name for each sample and clear instruction if various primers are to be used<\/li>\n<\/ul>\n
- \n
- Use the freezer in REID 310B marked \u2018Sequencing Samples\u2019 to transfer your samples and email biology+tech@montclair.edu<\/a>\u00a0when you leave samples<\/li>\n<\/ul>\n<\/div><\/div>Fragment analysis with the Applied Biosystems 3130 Genetic Analyzer<\/div>\n
- \n
- Quick Facts<\/dt>\n
- Mainly for population variance or progeny verification<\/dd>\n
- Keywords: RFLP, microsatellites, STR<\/dd>\n<\/dl>\n
Fragment analysis is an additional way to analyze genetic material. It has a wide range of applications such as:<\/p>\n
- \n
- Studying the variation of individuals in a\u00a0 population using amplified fragment length polymorphisms (AFLP).<\/li>\n
- Comparing known genetic profiles to identify individuals by examining microsatellites amplified by fluorescently tagged primer. Used by law enforcement to identify suspects also used in paternity testing (Heredity).<\/li>\n
- Analysis of single nucleotide polymorphisms (SNPs) using SNPlex or SNaPshot. Processes generally used to identify single base pair mutations at a specific site, like cancer or genetic condition screenings.<\/li>\n<\/ul>\n
Pricing can be drastically different depending on the needs of the associated project, please contact biology+tech@montclair.edu<\/a>\u00a0for further information.<\/p>\n
Example of Data<\/h2>\n
- Keywords: RFLP, microsatellites, STR<\/dd>\n<\/dl>\n
- Use the freezer in REID 310B marked \u2018Sequencing Samples\u2019 to transfer your samples and email biology+tech@montclair.edu<\/a>\u00a0when you leave samples<\/li>\n<\/ul>\n<\/div><\/div>
- Clearly indicate a unique name for each sample and clear instruction if various primers are to be used<\/li>\n<\/ul>\n
- If you are setting up your own sequencing (ONLY for PCR) pre-mix. Use 1.5\u00b5L PCR, 1.5\u00b5L single Primer (At concentration of 10\u00b5M<\/em>) and 7\u00b5L water for a total of 10\u00b5L<\/li>\n<\/ul>\n
- Provide a labeled tube for the PCR product or plasmid (label plasmid tube with conc. in ng\/\u00b5L<\/em>) and a labeled tube for the forward and\/or reverse primer (do not combine primers<\/em>). Primer concentration should be 10\u00b5M<\/li>\n<\/ul>\n
- Email biology+tech@montclair.edu<\/a>\u00a0a gel pic with the indicated bands and a positive and negative control if possible<\/li>\n<\/ul>\n
- Keywords: PCR product, plasmid<\/dd>\n
- The cutting edge next level of genetic sequencing to generate meta-data<\/dd>\n
![\"aflp](\"\/biology\/wp-content\/uploads\/sites\/43\/2019\/06\/aflpITSprimers.jpg\")
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